Analysis Note

ControlPOSITIVE CONTROL: colon cell line HCT116

Application

Anti-APC Antibody, C-terminus, clone C-APC 28.9 is a Mouse Monoclonal Antibody for detection of APC also known as Adenomatous Polyposis Coli Protein & has been validated in IF, IP, WB & IHC.

Western blot

Immunohistochemistry (Frozen sections)

Immunofluorescence

Immunoprecipitation

Optimal working dilutions must be determined by end user.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The adenomatous polyposis coli tumor suppressor gene is mutated (often deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and over 80% of colorectal cancers. C-APC 28.8 can be used for APC expression and detection of APC mutations.

Immunogen

Epitope: C-terminus

Human APC C-terminal fusion with MBP (maltose binding protein).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

feature_concentration_valuePlease refer to lot specific datasheet.

Physical form

Purified immunoglobulin. Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide.

Format: Purified

Quality

Tested

Specificity

Recognizes human APC, molecular weight is approximately 310kDa.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.